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The helix 69 (H69) RNA hairpins from E. coli are shown. The Ψm3ΨΨ and UUU RNA constructs are derived from positions 1906–1924 of E. coli 23S rRNA. The dsH69 RNA contains the stem-region of H69. The structures of pseudouridine (Ψ), 3-methylpseudouridine (m3Ψ), and uridine (U) are shown.