FIGURE 6.
The 1D imino proton (uridine H3, guanine H1, and pseudouridine N1H and N3H) NMR spectra of E. coli H69 RNA (Ψm3ΨΨ) are shown. The spectra in panel A were obtained at 15°C and pH 5.5 (upper) or pH 6.5 (lower). The spectra in panels B and C were obtained at 4°C, 10°C, 15°C, 25°C, and 37°C at pH 5.5 (B) or pH 6.5 (C). The buffer employed was 30 mM NaCl, 10 mM sodium phosphate, and 0.5 mM Na2EDTA in 90% H2O and 10% D2O. Nucleotides are assigned based on 1D NOE spectroscopy (Meroueh et al. 2000; Chui et al. 2002).