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. 2008 Apr;14(4):782–792. doi: 10.1261/rna.779908

FIGURE 6.

FIGURE 6.

The 1D imino proton (uridine H3, guanine H1, and pseudouridine N1H and N3H) NMR spectra of E. coli H69 RNA (Ψm3ΨΨ) are shown. The spectra in panel A were obtained at 15°C and pH 5.5 (upper) or pH 6.5 (lower). The spectra in panels B and C were obtained at 4°C, 10°C, 15°C, 25°C, and 37°C at pH 5.5 (B) or pH 6.5 (C). The buffer employed was 30 mM NaCl, 10 mM sodium phosphate, and 0.5 mM Na2EDTA in 90% H2O and 10% D2O. Nucleotides are assigned based on 1D NOE spectroscopy (Meroueh et al. 2000; Chui et al. 2002).

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