FIGURE 1.
Schematic picture of a tRNA molecule with positions that are modified by enzymes that appeared in our screen. N1-methylguanosine [m1G9], N4-acetylcytidine [ac4C12], N2,N2-methylguanosine [m2 2G26], N6-isopentenyladenosine [i6A37], N7-methylguanosine [m7G46], dihydrouridine [D47], and pseudouridine [Ψ26–28, 34–36, 65, 67] modifications are catalyzed by Trm10p, Tan1p, Trm1p, Mod5p, Trm8/82p, Dus3p, and Pus1p, respectively. Pseudouridine is shown in gray, other modifications in black. Position 26 can be modified by either Pus1p or Trm1p, depending on whether it is a uridine or a guanosine.