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NMIA probing of the guanine riboswitch aptamer domain in the absence (A) and presence (B) of 10 μM hypoxanthine for quantifying the melting behavior of the RNA with nucleotide resolution. The RNA was probed in the presence of 6 mM MgCl2. Numbers to the side of the gels denote the nucleotide position, and the bars correspond to the nonhelical regions of the RNA.
