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MPTP treatment leads to protein tyrosine nitration of specific proteins in the mouse midbrain. Two-dimensional, chemiluminescence-enhanced anti-nitrotyrosine immunoblots of ventral midbrain tissue of C57/bl mice 3 hr after injection of either saline (A) or MPTP (B). MPTP injection resulted in nitration of selective proteins. Indicated by the arrow is a protein with the physical characteristics of mouse tyrosine hydroxylase listed in protein databases.
