Figure 1.
Effect of 8-cyclopentyltheophylline on the CCPA- and Cl-IB-MECA-induced cardioprotective effect. Cultured ventricular cells were prepared and exposed to either CCPA (10 nM) or Cl-IB-MECA (10 nM) in the presence and absence of varying concentrations of the adenosine A1 receptor antagonist 8-cyclopentyltheophylline. Media were changed to that lacking agonist or antagonist. Cells were then exposed to room air at 37°C for 10 min before being exposed to simulated ischemia for 90 min. The percentage of cells killed and the amount of creatine kinase released were determined after the 90-min ischemic period. Data represented the means and standard errors of four experiments.