Figure 2.
Effect of MRS1191 on the CCPA- and Cl-IB-MECA-induced cardioprotective effect. Cultured ventricular cells were prepared and exposed to either CCPA (10 nM) or Cl-IB-MECA (10 nM) in the presence and absence of varying concentrations of the adenosine A3 receptor antagonist MRS1191. Media were replaced with fresh media lacking the agonist or the antagonist. Cells were then exposed to normal O2 for 10 min before being exposed to 90 min of simulated ischemia. The percentage of myocytes killed (A) and the amount of creatine kinase released (B) were determined after the 90-min ischemic period. Data represented the means and standard errors of four experiments.