Figure 1.

(A) Analysis of the deduced amino acid sequence of CaMNT1 of C. albicans CAI4 and comparison with S. cerevisiae sequences ScMnt1p and ScKtr1–3p using Seqnet GCG program. Positions of complete identity are indicated with asterisks and by full stops where three or more residues are conserved between any of the five sequences. Values for percentage similarities (parentheses) and identities between CaMnt1p and the other genes are given. (B) Hydrophobicity analysis (34) with a window of nine amino acids indicated a single transmembrane spanning region (box in A). (C) Northern analysis of total RNA obtained from C. albicans strains CAI4 and NGY24, respectively, hybridized with radiolabeled CaMNT1 probe. rRNA bands visible on ethidium bromide-stained gels (not shown) were used both as a loading control and molecular weight markers.