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Expression and signaling function of constitutive chimeras. (A) BaF3 cell lines transduced with the indicated expression constructs were analyzed by immunoblotting with anti-N-terminal EPOR antiserum. (B) To verify the integrity of the signaling function of these receptors, the indicated cell lines were rested or exposed to EPO (50 units/ml, 10 min) followed by preparation of nuclear extracts and electrophoretic mobility shift assay for STAT DNA-binding activity.
