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CCR-3 tropism maps to the V1 and V2 hypervariable regions of gp120. The pNL-Luc-E-R- HIV-1 indicator construct was pseudotyped with the indicated wild-type and chimeric HIV-1 envelope proteins and the resultant virions used to infect 293T cells expressing CD4 alone or together with the CCR-5 or CCR-3 chemokine receptor. The resultant luciferase activity is indicated in relative light units.
