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Restriction map of the esaI/esaR locus (A). The direction of transcription of esaI and esaR is indicated by the arrows. As indicated, a Tn5 insertion in the coding sequence of esaI generates mutant strain ESN51 (B); a HpaI–PstI deletion in the esaR gene is the basis for generating mutant strain ESΔR (C); a KpnI deletion in esaI/esaR becomes the basis for generating the mutant strain ESΔIR (D). The stippled region indicates where the coding segments of the two genes overlap.
