Figure 1.

Kv1.1 RNA level decreases with elevation of intracellular cAMP. (A) A representative RPA gel showing single samples at 6 and 24 h for control, forskolin, and Iso/IBMX-treated cultures. Undigested, full-length probes (Kv1.1 and cyclophilin) are indicated. Protected bands that were quantitated are boxed in the third lane. The mouse Kv1.1 probe protected two bands in rat-derived C6 glioma. Cyclophilin consistently gave a single band with a smear below. The largest cyclophilin band was used to quantify changes in Kv1.1 RNA levels. (B) Kv1.1 RNA levels in C6 glioma were treated with vehicle (control) or Iso/IBMX for up to 72 h. Triplicate plates were collected for each treatment group. Cyclophilin levels were unchanged over 12 h but declined in both treated and control samples collected at 24, 48, and 72 h. Kv1.1 levels did not change significantly over 72 h in control samples. Therefore, the data have been corrected to the cyclophilin levels at 0 h. Statistical analysis included a one-way ANOVA followed by a Tukey’s multiple comparison test.