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Elevation of cAMP decreases Kv1.1 protein. Cells were treated with vehicle (control) or Iso/IBMX for the indicated times, and a crude membrane fraction was prepared. Western blot analysis (Upper) was performed with a polyclonal antibody that specifically recognizes Kv1.1 protein (21). Equal amounts of protein were loaded per lane as evidenced by a prominent 51-kDa band of a Coomassie-stained paired gel (Lower).
