Table 2.
Protection of live-attenuated V. cholerae vaccine strains from intraintestinal CTXφ transduction by rstR
Recipient strain* | CFU in intestinal homogenates†
|
|
---|---|---|
Total no. of recipients | Transductants | |
BAH-2(pHK3) | 3.7 × 105 | 0 |
BAH-2(pBR322) | 5.6 × 105 | 5.3 × 104 |
O395-N1(pHK4) | 2.2 × 106 | 0 |
O395-N1(pACYC184) | 1.9 × 106 | 8.1 × 104 |
V. cholerae CTXφ donor strain LAC-1(pCTX-Kn) was mixed with each of the different recipient strains and gastrointestinally inoculated into suckling mice.
Intestinal homogenates were plated on selective media to enumerate the total numbers of colony-forming units (CFU) of each recipient strain and the numbers of kanamycin-resistant CFU (tranductants) of each recipient strain after 20 hr of intraintestinal growth. There were at least eight mice in each group, and the median number of CFU in each group is presented.