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Specific binding of VirD2 to GST–CyP fusions in vitro. An equal amount of total bacterial lysate expressing His-tagged VirD2 was incubated with buffer alone (lane 1) or with an equal volume of total bacterial lysate expressing GST (lane 2), GST–Roc1 (lane 3), or GST–CypA (lane 4). GST or GST fusion proteins were affinity purified with G-beads and subjected to SDS/PAGE and Western blot analysis.
