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. 1998 Jun 9;95(12):7040–7045. doi: 10.1073/pnas.95.12.7040

Figure 2.

Figure 2

Specific binding of VirD2 and GST–CyP fusions in the presence of detergent and high salt. An equal amount of total bacterial lysate expressing His-tagged VirD2 was incubated with an equal volume of total bacterial lysate expressing GST–Roc1 (lanes 1–4) or GST–CypA (lanes 5–8). Lanes 1 and 5, no additional salt or detergent added to the binding buffer; lanes 2 and 6, 0.5 M NaCl; lanes 3 and 7, 1 M NaCl; and lanes 4 and 8, 0.1% SDS. GST fusion proteins were affinity purified with G-beads and subjected to SDS/PAGE and Western blot analysis. (A) Coomassie blue stained SDS/PAGE. (B) Immunoblot with rabbit anti-VirD2 antibody.