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. 1998 Jun 9;95(12):7040–7045. doi: 10.1073/pnas.95.12.7040

Figure 4.

Figure 4

Delineating the CyP-interacting domain of VirD2. A series of deletions of the virD2 gene were generated by PCR amplification, subcloned into pEG202, and subjected to yeast two-hybrid analysis with cDNA clones of Roc1 and CypA in pJG4-5. A positive interaction was scored if EGY48 cells carrying a combination of pEG202 and pJG4-5 plasmids stained blue on X-Gal medium and grew in leucine-free medium in the presence of galactose but not glucose. Boxes represent the VirD2 region present in pEG202. The CyP interacting domain is indicated by the bar. The endonuclease (4), NLSs (6), and omega domains (31) are indicated. The rectangle within the endonuclease domain denotes critical residues essential to endonuclease activity (8, 52). Results of the yeast two-hybrid analysis are shown to the right of the figure.