Table 1.
Arabidopsis CyPs interact with VirD2 in the yeast two-hybrid assay
| cDNA clone (in pJG4-5) | Protein encoded | GenBank match (accession no. and ref.) | Interaction with LexA fusion to
|
||
|---|---|---|---|---|---|
| VirD2 (pEG202D2) | VirE2 (pEG202E2) | Bicoid (pRFHM1) | |||
| pJG4-5 | Vector only | − | − | − | |
| pD2-130 | Roc1 | L14844 (27) | + | − | − |
| pD2-294 | Roc4 | L14845 (27) | + | − | − |
| pD2-342 | CypA/Cyp1 | U07276 (28) | + | − | − |
The two-hybrid assay was done in yeast strain EGY48, which contains two reporter genes (LEU2 and lacZ) (10). A positive interaction was scored if EGY48 cells carrying a combination of pEG202 and pJG4-5 plasmids stained blue on X-Gal medium and grew in leucine-free medium in the presence of galactose but not glucose.