Viable E. coli and S. typhimurium actively secrete the signaling molecule. The response of the V. harveyi reporter strain BB170 (sensor 1−, sensor 2+) to a signaling substance produced and secreted by E. coli AB1157 and S. typhimurium LT2 but not E. coli DH5α is shown. V. harveyi reporter strain BB170 was diluted 1:5,000 in AB medium and light output per cell was monitored during growth. At the start of the experiment, either 1 × 106 E. coli AB1157, S. typhimurium LT2, or E. coli DH5α washed and resuspended viable cells (empty bars) or UV-killed cells (filled bars) was added. The data are presented as the fold-activation above the endogenous level of luminescence expressed by V. harveyi BB170 at the 5-hr time point. S.t, S. typhimurium; E.c, E. coli. Results for replicates were within 10%.