Restriction map of the DNA fragment from P. fluorescens WCS365, which includes the 5-kb HindIII fragment that complements the colonization defect. (A) The 5-kb HindIII fragment in which arrows indicate the direction and the size of the six ORFs. (B) The fragments used for complementation or single strand-DNA sequencing. B, BamHI; C, ClaI; E, EcoRI; Ev, EcoRV; H3 HindIII; K, KpnI; S, SmaI; Sa, SalI.