Table 1.

Influence of the presence of pTiA6 on the capacity of A. tumefaciens UIA143 to function as recipient in virB-mediated transfer of the RSF1010 derivative  pJB31

[AS]†, μM	Recipient Ti status	Total number of output*
		Donor, 108	Recipient, 108	Transconjugants	Transconjugants/output recipient ± SD‡
0	−	24.5	7.6	0	0
0	+	26.5	8.1	0	0
200	−	5.1	12.4	<2	(0.8 ± 0.8) 10−9
200	+	5.5	6.8	49,866	(7.3 ± 1.5) 10−5
400	−	3.8	13.6	12	(8.4 ± 3.5) 10−9
400	+	3.9	5.7	112,622	(2.0 ± 0.8) 10−4
800	−	1.9	13.2	20	(1.5 ± 0.6) 10−8
800	+	1.4	5.0	361,422	(7.2 ± 0.7) 10−4

^*Donor, recipient, and transconjugants in mating mix after 3-day incubation. 

^†Concentration of AS (μM) in the conjugation medium. 

^‡Data represent means (±SD) of triplicates from a single experiment. Three independent experiments with similar results were performed. Ratios between input donor [A. tumefaciens A348 (pJB31)] and input recipients were 25:1 for strain UIA143 and 7:1 for UIA143 (pTiA6).