Table 3.

Frequencies of pJB31 transfer from various virB mutant strains serving as donors in conjugations in which the recipient carries an intact virB operon

Donor strain (pJB31)	Gene function disrupted	Transconjugants/output donor*	% of A348 value
A348	None	(8.6 ± 0.22) 10−6	100
PC1000	virB1	(4.43 ± 2.80) 10−7	5.15
Other mutant strains tested:
PC1002, 1003, 1004, 1005, 1006, 1007, 1008, 1009, 1010, 1011, At12506	virB2, virB3, virB4, virB5, virB6, virB7, virB8, virB9, virB10, virB11, virD4	No transconjugants recovered†	0

Donor strains and the gentamicin-resistant recipient strain, A348(pJB61), were mixed at a ratio of 1:5; conjugation took place on induction medium (250 μM AS) for 3 days at 25°C. 

^*Data represent means of triplicates (±SD) from a single experiment. A total of three independent experiments were performed, each showing the same basic result. 

^†Even at concentrations of 800 μM AS, no transconjugants were recovered.