Figure 2.
Immunoprecipitation of dopamine receptor binding sites by D2S and D2L antibodies. (a) Antibody concentration-dependent immunoprecipitation demonstrated by [3H]spiperone binding in striatal tissues. Saturation point at 20 μl shows that both antibodies are equally sensitive. (b) Quantitative immunoprecipitation from substantia nigra, motor cortex, and striatum showing different amounts of D2S and D2L receptors. The results were confirmed with two different dopamine D2 receptor antagonists.