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. 1998 Jun 9;95(12):7069–7073. doi: 10.1073/pnas.95.12.7069

Figure 2.

Figure 2

Disruption of the COS1 gene from C. albicans. (A) Schematic showing the replacement strategy. Restriction sites are H (HindIII), Bg (BglII), X (XhoI), N (NcoI), and E (EcoRI). (B) Southern blot of genomic DNA to detect replacement of COS1 with hisG-URA3-hisG. After digestion of genomic DNA with BglII, the replacement event was detected by probing with a PCR product generated from CA13 and CA15 primers. Lanes: 1, CA I4 (wild-type parent); 2, LAC13 (Δcos1∷hisG-URA3-hisG/COS1); 3, LAC15 (Δcos1∷hisG/COS1); 4, LAC18 (Δcos1∷hisG/Δcos1∷hisG-URA3hisG); 5, CA I4 (wild type); 6, LAC13 (Δcos1∷hisG-URA3-hisG/ COS1); 7, LAC14 (Δcos1∷hisG/COS1); 8, LAC17 (Δcos1∷hisG/ Δcos1∷hisG-URA3-hisG); 9, LAC16 (Δcos1∷hisG/Δcos1∷hisG-URA3hisG).