Figure 4.

(A and B) Recombinant integrins render CHO cells permissive to SNV and NY-1 hantaviruses. The ability of SNV (strain CC107) and PHV hantaviruses to infect CHO cells or CHO cells transfected with the α_IIbβ₃ integrin (CHO-A5 cells) or the α_vβ₃ integrin (CHO-VNRC) are depicted at 7 days postinfection (A). Representative pictures were taken from identically stained uninfected or hantavirus-infected cells (200 X magnification). The nucleocapsid protein present in infected cells was immunoperoxidase stained (dark brown precipitate) as described (27). (B) Quantitation of NY-1, SNV, and PHV hantavirus infection of CHO and CHO-integrin cell lines was determined 36 hr postinfection. The fold increase in the number of infected cells compared with immunoperoxidase-stained CHO cells is presented. Results were reproduced in six separate experiments.