Figure 3A–B.

Low power back-scattered detector (BSD) micrographs and electron microprobe analysis areas: Fig. 3A is a BSD image of an Ae2_a,b^+/+ specimen cut through the maturation stage incisor enamel and that from the Ae2_a,b^−/− specimen is shown in Fig. 3B. The low contrast in the Ae2_a,b^−/− enamel compared to the Ae2_a,b^+/+ specimen indicates low level of mineralisation. Fig. 3C is of an Ae2_a,b^+/+ first molar and Fig. 3D of an Ae2_a,b^−/− specimen. Note that the difference in electron density between the Ae2_a,b^+/+ (Fig. 3C) and Ae2_a,b^−/− molar enamel (Fig. 3D) is less pronounced than that seen in the incisors (cf. Fig. 3A & B above). All the images were acquired under identical microscope parameters. The areas analysed for mineral content in enamel, dentine and bone are shown by dots. (Bars=300μm).