FIGURE 10.

CXCL11 up-regulates ICAM-1 on bronchial epithelial cells but does not increase LFA-1:ICAM-1 mediated adhesion of lymphocytes to the bronchial epithelium. A and B, FACS analysis of ICAM-1 expression on bronchial epithelial cells after stimulation with CXCL11 for 30 min (A) and 120 min (B). Dotted lines show epithelial cells labeled with secondary Ab alone. Thick line, no CXCL11; thin line, 100 ng/ml; dashed line, 400 ng/ml. One experiment representative of three similar experiments is shown. C, FACS analysis of ICAM-1 expression on bronchial epithelial cells, after stimulation with CXCL11, and normalized to unstimulated control cells: no CXCL11 white bars, 100 ng/ml gray bars, and 400 ng/ml black bars. The results are shown as the average and SD from three similar FACS experiments. The p values were calculated using a Student's t test. *, p = 0.05; **, p < 0.01. D, Adhesion of T lymphocytes to the bronchial epithelium is LFA-1: ICAM-1 dependent but is not increased by various concentrations of CXCL11. T cells adhesion to the bronchial epithelium was measured in the presence of increasing amounts of CXCL11 and various blocking mAbs directed against LFA-1 on the lymphocyte, or ICAM-1, ICAM-2 and VCAM-1 on the epithelium: no CXCL11, white bars; 100 ng/ml, gray bars; and 400 ng/ml, black bars. The results are shown as the average and SD from three similar experiments. The p values were calculated using a Student's t test. *, p = 0.05; ***, p < 0.005.