FIGURE 6.

Bronchial epithelial cells express CXCR3 and undergo actin rearrangements in response to CXCL11. A, FACS analysis of CXCR3 expression on bronchial epithelial cells released with EGTA (thin line) or polyvinylpyrrolidine-EGTA-trypsin (thick line), and on T cells (dashed line). Bronchial epithelial cells incubated with secondary alone are shown as dotted control. Results are representative of four different experiments. B–G, Incubation of bronchial epithelium with 100 ng/ml CXCL11 at various time points 0 min (B), 5 min (C), 15 min (D), 30 min (E and G), and 60 min (F) causes an increase in actin polymerization and stress fiber formation that is inhibited by preincubation of the epithelium with a blocking mAb against CXCR3 (G). Scale bar, 10 μm. One representative experiment of three is shown. H, Transepithelial migration is inhibited by blocking mAbs against CXCR3 on the T cell, but not against CXCR3 on the epithelial cell. Transepithelial migration assay after 120 min in response to 400 ng/ml CXCL11. T cells or epithelial monolayers were preincubated with Abs to CXCR3 or LFA-1 before being washed and used in a migration assay. Data are expressed as mean ± SD of triplicates, and one representative experiment of three is shown. The p values were calculated using a Student's t test. **, p < 0.01.