Figure 1.

The presence of α_1E antisense ONs in the culture medium decreases the R-type current amplitude. (A) Activation of Ba²⁺ currents with various depolarizing pulses (V_test = −60, −40, −20, and 0 mV from a V_hold = −80 mV) in untreated cells, cells cultured in the presence of 4 μM α_1E antisense ON, and cells treated with 4 μM α_1E sense ON. Data pooled from 3 to 4 cells. R-type currents were measured in the presence of toxins to block L-, N-, and P/Q-type current components (see Methods). (B) Current-voltage relationship averages for untreated cells (circles, n = 6), sense α_1E ON (squares, n = 5), and antisense α_1E ON (triangles, n = 11). Currents from cells treated with antisense ON were significantly smaller than the untreated (P < 0.01) and sense-treated controls (P < 0.03). (C) Peak current distribution in the untreated, antisense α_1E, and sense α_1E-treated cell groups.