Figure 3.

Antisense ONs against α_1A do not affect the expression of R-type currents but decrease the PQ + R components. (A) Activation of R-type Ba²⁺ currents with depolarizing pulses from V_hold = −80 mV to V_test = −60, −40, −20, and 0 mV in cells cultured in the presence of either 4 μM α_1A sense or α_1A antisense ON. Data pooled from five cells. (B) Addition of α_1A antisense ON decreased the P/Q + R component compared with cells grown in the presence of α_1A sense ON. Ba²⁺ currents were elicited with depolarizing pulses from V_hold = −80 mV to V_test = −60, −40, −20, 0, and +10 mV, data pooled from five cells.