Figure 7.

Effects of ICI (100 nm) on the E₂-induced changes in [Ca²⁺]_i oscillations. A, Cells were treated with ICI for the entire period of the experiment as indicated by the white bar on the top, and E₂ was applied for 10 min starting 20 min after the initiation of ICI treatment (stippled bar on the top). E₂ induced increases in the frequency of [Ca²⁺]_i oscillations and synchronizations in the presence of ICI. B, ICI alone did not cause any significant changes in [Ca²⁺]_i oscillations and synchronization. Thirteen of 30 and eight of 32 LHRH-1 neurons from cultures treated with ICI plus E₂ and ICI alone, respectively, are shown.