Skip to main content
. Author manuscript; available in PMC: 2008 Mar 24.
Published in final edited form as: Biochemistry. 2007 Nov 13;46(49):13948–13960. doi: 10.1021/bi7011977

Table 3.

Mg2+-dependence of hairpin RNA substrate folding

RNA substrate [Mg2+]1/2 (µM)a ha
HP6 35 ± 4 1.21 ± 0.11
     
HP14 82 ± 10 1.15 ± 0.17
     
HP21 68 ± 4 1.16 ± 0.05
a

Determined by nonlinear regression of hairpin RNA substrate fluorescence versus Mg2+ concentration at 25 °C using equation 3. Values are quoted as the mean ± SD for five independent experiments.