Figure 6.
Representative images with high magnification inserts of Griffonia simplicifolia isolectin B4 staining for microglia activity in the suprapyramidal blade of the dentate gyrus of wildtype (WT) and IL-1R1 knockout (IL-1R1−/−) mice. In saline controls, minimal staining of microglia with thin ramified processes was seen in both the WT (A) and IL-1R1−/− (B) mice. At 24 hrs post-TMT (2.0 mg/kg i.p.), increased staining of lectin+ microglia displayed ramified microglia with shortened, thick processes in both WT (C) and IL-1R1−/− (D) mice. By 72 hrs post-TMT, reactive and activated microglia with an amoeboid phagocytic morphology were evident in both WT (E) and IL-1R1−/− (F) mice. Scale bar = 100µm.