Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Q-RT-PCR primer sequences and concentrations
| Gene | Forward Primer | Reverse Primer |
|---|---|---|
| TNFα | TGGCCTCCCTCTCATCAGTT | GCTTGTCACTCGAAATTTGAGAAG |
| (300nM) | (900nM) | |
| IL-1α | TCGGGAGGAGACGACTCTAA | GGCAACTCCTTCAGCAACAC |
| (600nM) | (600nM) | |
| IL-1β | TGGTGTGTGACGTTCCCATT | CAGCACGAGGCTTTTTTGTTG |
| (300nM) | (300nM) | |
| IL-1ra | CTCCTTCTCATCCTTCTGTTTCATT | TGCATCTTGCAGGGTCTTTTC |
| (300nM) | (300nM) | |
| MyD88 | ACTGGCCTGAGCAACTAGGA | TGTCCCAAAGGAAACACACA |
| (300nM) | (300nM) | |
| GAPDH | GGGAAGCTCACTGGCATGG | CTTCTTGATGTCATCATACTTGGCAG |
| (200nM) | (200nM) |
