Table 1.
RNAi constructs
| Gene (name) | Sequence | RNAi strain | Description |
|---|---|---|---|
| age-1 | B0334.8 | II-7J02 | Ahringer library |
| daf-2 | Y55D5A.5 | pSH510 | From S. Henderson |
| daf-16 | R13H8.1 | pSH508 | From S. Henderson |
| gfp | Green fluorescent protein | pPD128.110 | From A. Fire |
| ev | None | pPD129.36 | Empty vector (A. Fire) |
| hyl-2 | K02G10.6 | X-2D18 | Ahringer library |
| lagr-1 | Y6B3B.10 | I-6P22 | Ahringer library |
| tram-1 | C24F3.1 | IV-5K16 | Ahringer library |
| hyl-1 | C09G4.1 | pJW9 | hyl-1 seq spanning Lag1p motif* |
| pUC18 | None | pUC18 | Classic pUC18, control for pJW9 |
*hyl-1 nucleotides 486–639 between T7 and T3 promoters in pUC18 plasmid