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. 2007 Nov 21;38(4):455–462. doi: 10.1165/rcmb.2007-0326OC

Figure 5.

Figure 5.

Cytokine mRNA expression of secondary granulomas from nonvaccinated guinea pigs aerosol-challenged with M. tuberculosis. Expression of IFN-γ, TGF-β, TNF-α, IL-12p40, and iNOS mRNA were measured from secondary granulomas microdissected from the upper left lung lobes of nonvaccinated guinea pigs 6 weeks after virulent challenge. Values for Ct were converted to “expression levels” to allow for fold comparisons between samples, expression level = 2(40-Ct). Data were normalized to HPRT rRNA followed by values derived from nongranulomatous tissues and expressed as means ± SEM (n = 8–10 capfuls of microdissected tissue from the upper left lung lobes). Inset: comprehensive chart mapping the relative contributions of cytokine mRNA expression in secondary granulomas from the upper left lung lobes of nonvaccinated guinea pigs. The sum of the relative expression levels of IL-12p40 mRNA (green), TNF-α mRNA (yellow), TGF-β mRNA (blue), IFN-γ mRNA (red), and iNOS mRNA (gray) in the secondary granulomas microdissected from nonvaccinated guinea pigs was used to calculate the relative proportion of transcripts represented by each cytokine.