Fig. 4.
Phosphorylation of p47phox in neutrophils (3 × 106 cells/ml) activated by various stimulators. Lane 1; Methanol (0.5% of solution) was used in the control experiment (2 min). Lane 2; Three nanomolar 4β-phorbol-12β-myristate-13α-acetate (PMA) was used, and stimulation was stopped at 5 min. Lane 3; One micromolar N-formyl-methionyl-1-leucyl-phenylalnine (fMLP) was used, and stimulation was stopped at 2 min. Lane 4; Eight micromolar 1-palmitoyl-2-linoleoylglycerol hydroperoxide (PLG-OOH) was used, and stimulation was stopped at 2 min.