Figure 3.

HIV-1 gp120 induces cellular relocalization of PC-PLC in MDMs. Monocytes were seeded and cultured as described in “Methods.” At day 7, cells were treated with R5 gp120 (1 μg/mL) for the indicated periods of time (B-D) or left untreated (A). Some cultures were treated with D609 (50 μg/mL) for 30 minutes before gp120 exposure (F-H) or with D609 alone (E). Cells were then fixed, permeabilized, and stained with rabbit anti PC-PLC Abs (detected in white or in red) and DAPI (in blue) as described in “Methods.” PC-PLC expression and cellular localization were detected by CLSM analysis. The results from 1 representative experiment of 3 independently performed are shown. The bars correspond to 20 μm. Color panels show a single cell of the corresponding black and white panels at higher magnification.