Table 1.

Effect of D609 on CCL2 secretion induced by R5 AT-2–inactivated HIV-1 in MDMs

Treatments	CCL2, pg/mL
	Donor 1	Donor 2	Donor 3
None	5362	1230	3452
R5 AT-2-HIV-1	11 328	3416	15 254
D609	3790	875	1970
D609+ R5 AT-2-HIV-1	5760	1150	3520

Monocytes were seeded in 96-well cluster plates (2×10⁵ cells/0.2 mL per well). After 7 days, culture medium was removed and replaced with 0.2 mL fresh medium. MDMs were pretreated with D609 (50 μg/mL) or left untreated. After 30 minutes, some cultures were exposed to R5 AT-2–inactivated viruses (17 ng HIVp24^gag/10⁶ cells). After 24 hours of culture, supernatants were harvested and frozen before CCL2 determination. Data are the mean values of duplicate samples. The intersample variability did not exceed 10%.