Table 2.

Effect of TPCK on CCL2 secretion induced by R5 gp120 in MDMs

Treatment	CCL2, pg/mL
	Donor 1	Donor 2	Donor 3
None	4600	29 022	30 000
R5 gp120	22 200	90 260	98 400
TPCK	2500	15 000	19 300
TPCK+R5 gp120	5800	12 000	16 250

Monocytes were seeded and cultured as described in the legend to Table 1. MDMs were pretreated with TPCK (10 μM) or left untreated. After 1 hour, some cultures were exposed to R5 gp120 (1 μg/mL). After 24 hours of culture, supernatants were harvested and frozen before CCL2 determination. Data are the mean values of duplicate samples. The intersample variability did not exceed 10%.