Table 1.

Sequences of oligonucleotides used in the present study

Name	Sequence (5′ to 3′)	Position in PROCR sequence (AF106202)
Primers used for cDNA amplification of PROCR genotyping
EPCR 2347 Fr	CATTGCTGCCGATACTGCTGC	nt 2347 to 2367
EPCR 4993 Fr	CCCAGACACCAACACCACG	nt 4993 to 5010
EPCR 6970 Rv	AAGATGCCTACAGCCACACC	nt 6970 to 6951
EPCR 7320 Rv	GTCTGTCTTTGGAGGATGGGCC	nt 7320 to 7299
EPCR 7190 Rv	CAAGTACTTTGTCCACCTCTCC	nt 7190 to 7169
EPCR 3960 Fr	GAGGCAGAGCAATCCTGACA	nt 3960 to 3980
EPCR 4562 Rv	CATCTCCTCTTCCACTGCCC	nt 4526 to 4507
EPCR 4069 Fr	GGGCCAGGTCTCTAGCAGCC	nt 4069 to 4088
Primers used to clone the EPCR cDNA in the pCI-neo expression vector
First-round PCR
EPCR upstream	GGTCCGGAGCCTCAACTTCAG	nt 2315 to 2335
EPCR downstream	ACTTTCCCTTGCCAGCCTCC	nt 7054 to 7035
Nested PCR with oligonucleotides containing the cloning sites
EPCR Nhe IFr	GACTCACTATAGGGAGACCCAAGCTGgctagcTCAGGATGTTGACAACATTGCTGCCGATAC
EPCR Not IRv	GGTGTTTCAGTTGGGGAGTCTCGAgcggccgcGAGCTGAAACTTTCCCTTGCCAGCCTCCATC	nt 7062 to 7032
Primers used to clone the cDNA of EPCR 1–209 (rEPCRsol) in the pCI-neo expression vector
pCI-neo upstream 2	CGAGACAGAGAAGACTCTTGCGTTTC	[in plasmid pCI-neo sequence]
EPCRsol1–209NotI	CACAAGGAAAAGGTATTAgcggccgcTTAAGTGTAGGAGCGGCTTGTTTGGCTC	nt 6908 to 6884
Primers used to clone the cDNA corresponding to EPCR isoform (rEPCRisoform) in the pCI-neo expression vector
5′ part of the cDNA
pCI-neo upstream 2	CGAGACAGAGAAGACTCTTGCGTTTC	[in plasmid pCI-neo sequence]
EPCR 6568Rv	CCCGCAGTTCATACCGAGTG	nt 6568 to 6549
3′ part of the cDNA
EPCR 4993Fr	CCCAGACACCAACACCACG	nt 4993 to 5012
EPCR 7480 Rv Not I	CACAAGGAAAGGTATTAgcggccgcGGGATTTTACATTTCCACCACTTCTTCGTG	nt 7480 to 7451

Restriction site sequences are shown in lowercase letters. The sequences complementary to the EPCR cDNA are in bold letters.