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. Author manuscript; available in PMC: 2008 Mar 25.
Published in final edited form as: J Biol Chem. 1998 Jul 10;273(28):17917–17925. doi: 10.1074/jbc.273.28.17917

Fig. 12. GST pull-down of GKLF by Sp1.

Fig. 12

The GST pull-down experiments are described under “Experimental Procedures.” Western blot analysis was performed on various protein fractions using a polyclonal rabbit GKLF antibody (13). Lanes 1 and 2 contained input lysates from mock- (vector alone-) transformed and GKLF-transformed bacteria, respectively. Lanes 3–5 contained the flow-through (F.T.) fractions of GKLF that did not bind to the glutathione-Sepharose 4B beads containing GST, GST-Sp1ZnF, and GST-Sp1Q1, respectively. Lanes 6–8 contained the eluant (E.T.) fractions of GKLF that bound to the beads and were eluted with 1 mm reduced glutathione. The location of GKLF is indicated and has a molecular mass of 24 kDa. The asterisk denotes a nonspecific cross-reacting material that was present in the lysates of mock-transformed bacteria.