Fig. 4. Cotransfection of CHO cells with various CYP1A1 reporter and GKLF effector constructs.

Transfection conditions were essentially the same as those described in Fig. 3. The amount of reporter and effector plasmids used was 5 μg/10-cm dish each. A description of the reporter constructs can be found under “Experimental Procedures.” PMT3 indicates the vector alone. Shown are the means ± S.E. of six independent experiments, each of which was carried out in duplicate.