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. Author manuscript; available in PMC: 2008 Mar 25.
Published in final edited form as: J Biol Chem. 1998 Jul 10;273(28):17917–17925. doi: 10.1074/jbc.273.28.17917

Fig. 7. EMSA of BTE oligonucleotides using CHO cell nuclear extracts.

Fig. 7

EMSA was performed using 0.1 pmol of labeled BTE oligonucleotides and 10 μg of nuclear extracts obtained from CHO cells. Where indicated, competitor oligonucleotides (see “Experimental Procedures”) were added at a 10-fold molar excess of the labeled probe. In experiments involving antibodies, 2 μg of the stated antibody were added to the reaction. PI denotes pre-immune serum for GKLF. Super-shifted bands in lanes 7 and 8 are labeled with arrowheads. The positions of Sp1 and Sp3 in the DNA-protein complexes are indicated. The asterisks indicate either nonspecific complexes (since they competed poorly) or complexes containing as yet unidentified proteins.