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. 2007 Oct 12;407(Pt 3):435–450. doi: 10.1042/BJ20070579

Figure 4. DCF fluorescence measurement of hydrogen peroxide generation by metal–ligand complexes.

Figure 4

(A) Ligands were incubated with Cu(II) and hydrogen peroxide generation (redox activity) was determined by DCF fluorescence. All samples contained ascorbate and HRP. Ligand and/or metal were added as indicated. No ligand induced higher levels of hydrogen peroxide than Cu(II) alone indicating a lack of redox activity towards Cu(II). (B) Ligands were incubated with Fe(III) and hydrogen peroxide generation was determined by DCF fluorescence. All samples contained ascorbate and HRP. Ligand and/or metal were added as indicated. Several ligands induced a significant increase in hydrogen peroxide compared with Fe(III) alone (*P<0.001). phen, phenanthroline.