Skip to main content
. 2007 Oct 12;407(Pt 3):373–381. doi: 10.1042/BJ20070481

Figure 1. Repression of cAMP-dependent gene expression in Huh7 cells.

Figure 1

Human hepatocarcinoma Huh7 cells were infected with adeno-β-gal or adeno-hPXR at an MOI of 15. After 16 h of infection, the cells were washed with PBS and were incubated in serum-free medium in the presence or absence of rifampicin (10 μM) and cAMP (100 μM) for an additional 14 h. (A) Western blot analysis using cell lysates to determine the expression of hPXR. (B) Quantitative real-time PCR was performed as described in the Experimental section. Relative levels of mRNAs were expressed by taking the levels in DMSO-treated Huh7 cells as 1. Results are means±S.D. for three experiments.