Figure 1. Fetuin-B expression is induced by FXR agonists in human but not in mouse hepatocytes.

HepG2 cells (A), human primary hepatocytes (B) or mouse primary hepatocytes (C) were treated with either 50 μM CDCA or 5 μM GW4064 for 24 h. Total RNA was extracted and human fetuin-A, human fetuin-B and mouse fetuin-B mRNA levels were measured by real-time QPCR and normalized to 28S mRNA levels. Results are expressed as the fold change over the DMSO; results are means±S.E.M. (n=3). Statistical significance was assessed using a Student's t test. *P<0.05 compared with the control.