Table 1. Dissociation constants (Kd), association constants (Ka) and free energies of binding (ΔG) for the interaction of UL54 with DNA.
| Ligand | Length (nt) | Kd (mM) | Ka (M−1) | ΔG (J/mol) | Ligand structure |
|---|---|---|---|---|---|
| Promoter-30 | 30 | 48 | 21050 | −24660 | 5′-GGGAGCTCGTTTAGTGAACCGTCAGATCTC-3′ |
| Promoter-20 | 20 | 61 | 16420 | −24050 | 5′-GGGAGCTCGTTTAGTGAACC-3′ |
| Promoter-15 | 15 | 93 | 10720 | −22990 | 5′-GGGAGCTCGTTTAGT-3′ |
| Promoter-12 | 12 | 130 | 7681 | −22170 | 5′-GGGAGCTCGTTT-3′ |
| Promoter-10 | 10 | 115 | 8719 | −22480 | 5′-GGGAGCTCGT-3′ |
| Promoter-9 | 9 | 97 | 10320 | −22900 | 5′-GGGAGCTCG-3′ |
| Promoter-8 | 8 | 99 | 10070 | −22840 | 5′-GGGAGCTC-3′ |
| Promoter-7 | 7 | 95 | 10500 | −22940 | 5′-GGGAGCT-3′ |
| Promoter-6 | 6 | 94 | 10680 | −22980 | 5′-GGGAGC-3′ |
| Promoter-5 | 5 | No binding detected | 5′-GGGAG-3′ | ||
| Rv-Promoter-30 | 30 | 49 | 20450 | −24590 | 5′-GAGATCTGACGGTTCACTAAACGAGCTCCC-3′ |
| ds-Promoter-30 | 30 | 42 | 23580 | −24950 | 5′-GGGAGCTCGTTTAGTGAACCGTCAGATCTC-3′ 3′- CCCTCGAGCAAATCACTTGGCAGTCTAGAG-5′ |
| Random-30 | 30 | 61 | 16390 | −24040 | 5′-TCAGTAACTTAGCCTTGTCTTGAATCATCC-3′ |
| RNA-30 | 30 | 59 | 17010 | −24130 | 5′-GGGAGCUCGUUUAGUGAACCGUCAGAUCUC-3′ |