Figure 3. Inhibition of transcription in cells overexpressing SLTM.

(a) Total RNA synthesis. HeLa cells were transfected with 0.1 μg of either empty plasmid or pSLTM-HA and incubated for 48 h. The cells were then exposed to [³H]uridine (1 μCi/well) for 1 h before being lysed, harvested and the radioactivity measured. (b) mRNA synthesis. HeLa cells were treated as described above and poly(A)⁺ RNA isolated before measurement of radioactivity. (c) For comparison, inhibition of [³H]uridine incorporation was measured in HeLa cells exposed to different concentrations of STA. ●, Total cellular incorporation; ○, incorporation into mRNA. Values represent the means±S.D. for three separate experiments. *P<0.005; (Student's t test).