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. 2007 Oct 12;407(Pt 3):355–362. doi: 10.1042/BJ20070170

Figure 5. Induction of apoptosis in HeLa cells by SLTM, assessed by Hoechst staining.

Figure 5

HeLa cells were seeded into 60-mm-diameter dishes and transfected with 1 μg of pEYFP-C1 or pEYFP-SLTM. The cells were incubated overnight and then transferred to serum-free medium for 24 h. Next, cells were fixed and stained with Hoechst and assessed by fluorescent microscopy. (a) Fluorescence of cells transfected with pEYFP-SLTM; (b) Hoechst staining of the same field as that shown in (a) (the scale bar represents 15 μm); and (c) change in apoptotic index of HeLa cells after expression of SLTM, assessed by cell counting. This experiment was performed in triplicate (results are means±S.D.) and repeated three times. The results shown are those obtained in a typical experiment.